fruit fly ( Drosophila melanogaster )
dPERK

Western result: ++

GST GST-dPERK (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: dPERK
CBI Antibody Core Code: A0338
"Lot" Number: 1 Position: 100(816-915)
% Identical Homology of Target to Mouse Protein: 35% (NP_079976.1| phosphoglucomutase 1; phosphoglucomutase 2)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
DDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFDLKNIN
YSQHQLVSNSFQIESVRPKSSGSDDANDDNKARRKPLTLA
LAQNHNNNQNGSQPTPSSAT

Sequence Name: dPERK
Full name: eukaryotic translation initiation factor 2 alpha kinase
UniGene Number: N.A.
Accession Number: AF193340
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1162
NCBI information page: click here

Full length sequence:
MQDDLDGIVRHRRRSLSFLQIVTLTMAGLVAFDPAQVLAGHPTTDSELQT
AGSPRPPGLEHCVDQEERRVARRLLYISTLDGRLSALDIAKSGKLRWSVP
TGPGPLISSSIHRLELTNNGQFVRMIPSLSGGIYKFDGDSIDPIPITAEH
LLSSSAKFSDDLVISGGKETRSYGVSVRTGQLLYECSLNGCVNSTEEGLA
IDDTIREPDEEDQLEDGEQLRDEAGYIVRHDPLLDDVIIVRRQTQTVRAV
ESRTGVERWNFSVGQHELDLVRPSECQLQPRDELELAVLDVDIKVVVPEG
IICAFSKSEPQTMLWKYKFDHPIVSAWNTNADDELQPIDLFSSAQWLWDQ
DENDTELPNAPQSPPSIYLGMYDKQLYIQESIRLRQEIMDQTKVYQQLTG
DTSLMPRIPWKPISASSKSLVIFRKDQEDPEMIAEGAVAQGGELVPYDDE
NFAVAAQSVLNASEFVNGNGFYFYTTGDLNGPQECSTQNNPTDLPAITAP
TSPTNATSEGTEATGNHSVNDDLGFSLDDIDAPVKVVILSLWFWWKEIVV
IAFTSAVILNIFMGQRNQRVEREYLVIERHVPVQTAIEATEASTQALLGP
VVPMQRPGNRFSFPPGQANQRTISESTTDSGEHYTSRFQSDFELMQCLGR
GGFGVVFEAKNKLDENRYAIKRITLPNKESSRQRVLREARTLASCEHHNI
VRYFHSWTETPPTGWQEEEDRKLLAHELSTSIQIETPDDSTMPSLTEQLK
EKRQQQLLSWVSDAANSTACSHDFHLPGESSLKNIREEYDYDEEEDSLIE
FRSESQSAALRAEEEDDTDDDYEEDEEQQGDHEKRHRSSVSIDIHSASFD
LKNINYSQHQLVSNSFQIESVRPKSSGSDDANDDNKARRKPLTLALAQNH
NNNQNGSQPTPSSATILNGTVAKPSKVYLYIQMQLCRKESLRDWLRDNRS
ETRAAHIGDIFHQIVDAVDYVHLKGLIHRDLKPSNIFFSQDGQIKIGDFG
LVTDMADIPNLVAKCGDQSGLPSCARHTQQVGTHLYMSPEQLLGQHYDYK
VDIYSLGLIFFELHVYFSTEMERIKTLRSLRDGQYPKDFAVNYPQQYDLL
QQMLSAQPEQRPQTKQLKSQLRNILQLPHLLSEGQSEQAELAERARRLSR
SRTFSSSSEPHQ