fruit fly ( Drosophila melanogaster )
Rb1cc1

Western result: +

GST GST-Rb1cc1 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Rb1cc1
CBI Antibody Core Code: A0660
"Lot" Number: 1 Position: 100(215-314)
% Identical Homology of Target to Mouse Protein: 31% (NP_035844.1| bromodomain adjacent to zinc finger domain, 1B; Williams-Beuren syndrome chromosome region 9 homolog (human))
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
DFHGFDELLDNDDVFTAQQQPTPQQQALTESSQADSPNKK
LKMGDEVETESGEQTERGVQTLTSSSSLARRPNLNLLQWI
SSKGNHRALQLMSDECIQGL

Sequence Name: Rb1cc1
Full name: CG1347
UniGene Number: N.A.
Accession Number: NP_649573
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1264
NCBI information page: click here

Full length sequence:
MMLYVFHVDVGRMLSFDMNVALQPVEHLKETIQKLHNIPAANIVLLVSGG
EMLTHSTQVSCYSAGTDTNPIYMFLTGDERLPPTIYNSDADNELRRQVEE
SHRLPPALETVRQRAQLAQHMRELARKEEDQCERLVHEQHLQQQGWSAVV
ANMEDLTNEFCDRFQNFCSFFDRHLQKRESFLELLRNFPDDLKQLGRIPI
LPDLMSLAEADFHGFDELLDNDDVFTAQQQPTPQQQALTESSQADSPNKK
LKMGDEVETESGEQTERGVQTLTSSSSLARRPNLNLLQWISSKGNHRALQ
LMSDECIQGLDVFSVEIYEKLKEEVNQIIKLANQADVKEIKGLGDRLCKL
EEFKFRIKKMVQEQKEQALALQQNEARAQNLRDNSVLPDLCLSHRSQLQV
MLDNHIKIREYCRCIANSKDELGWNLHTRLRRIVWIENGMSEFDNRLLFN
HRCLRRAERHICIIEQIHRAPSTYVSAVAEVVRRKIFSDEFRQWATRLSA
DFDRIHSEELSRRRDFNASFEGHFLNILFPGMGDMPPAFANEHPLSFDTR
LPSLSRSDMELLSSQLPELAGQLQLPDMKPVIDFFALRSDKNVEPDQEQE
RDIPLMAPRLAELQAQAAASDCETDTETEFEKLSTTSKCVATSISTSLVE
QVAQSTATEDLLMLSAGTLTEENAGSFEKMRSDMESMTKLARACLAEARS
NVGVFRSDVANYQDELQSELHLLRDKCNVLKMMCEEREQKLQEKLDEMSL
KLQLAEQEKQAAVAQAREQLIHEHKTELESLRCRFKLMTSMERSPSDSSL
EKIERPTSSASVVSANAGVDIDHLLAQQRQELLAQRERAISEAVDSERSL
WQSRLLPLNSESVMANVGILKDMLADKERQLDQLREQNKILTQETYQLKT
RLEMITNEDGNSWLKEKIDYLNKDKCRLEEELSQEKSRRQEMESSVAAMR
SSAYDLNVGGPLTRSKSGVSSSHRSIALEGCARGDLVFVVWSMRHAQFMV
VQDSLTLYFVHADSLASLQLTAPTSPTPSLDPSQTVVDINQIPLPYYAIG
RVIDKEYCQARKDDNRYRVSRGSKFYRIKLAPLPSRNASRRERLESSSSI
AAVVASPRDVIDAPSSNTSVYQSFKQRTVSITSVNEEDDESASLLSERCR
YISRQELDQPAEAESTAPVAETSPNQTIQPEDPDPEPETITIYVPPNAPA
VLTATPSTRASILASISAKPIISTDSMLPMSIGTAAISEDSDEYRSLEGK
DDGDGDADDFPISE