fruit fly ( Drosophila melanogaster )
Tin; NK-4

Western result: +/-

GST GST-Tin; NK-4 (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Tin; NK-4
CBI Antibody Core Code: A0661
"Lot" Number: 1 Position: 100(60-159)
% Identical Homology of Target to Mouse Protein: 34% (NP_075633.1| non-POU-domain-containing, octamer binding protein; non-POU-domain-containing, octamer-binding protein)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
IDGAATASALFAAGEYQNPHQYLNHQQHQQSELPIPQQQL
HHQHLDDGATTSSSLSPLLPPPPHQLYGGYQDYGMPAHMF
QHHHGHPHQSFQHSASAYNM

Sequence Name: Tin; NK-4
Full name: MUSCLE-SPECIFIC HOMEOBOX PROTEIN TINMAN (MSH-2) (NK-4), Tinman
UniGene Number: N.A.
Accession Number: P22711
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 416
NCBI information page: click here

Full length sequence:
MLQHHQQQAQSGGYYDHYTQSPSPGSLTNADALNTTPFSVKDILNMVNQT
EAYEGSYGHIDGAATASALFAAGEYQNPHQYLNHQQHQQSELPIPQQQLH
HQHLDDGATTSSSLSPLLPPPPHQLYGGYQDYGMPAHMFQHHHGHPHQSF
QHSASAYNMSASQFYAGASATAYQTPATYNYNYAGSGEVYGGATPSAVDI
KSEYIPTPYVTPSPTLDLNSSAEVDSLQAPTQKLCVNPLSQRLMETASNS
SSLRSIYGSDEGAKKKDNSQVTSSRSELRKNSISGNSNPGSNSGSTKPRM
KRKPRVLFSQAQVLELECRFRLKKYLTGAEREIIAQKLNLSATQVKIWFQ
NRRYKSKRGDIDCEGIAKHLKLKSEPLDSPTSLPPPIPNHVMWPPTMQQS
QQQQQHHAQQQQMQHM