fruit fly ( Drosophila melanogaster )
coracle

Western result: +

GST GST-coracle (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: coracle
CBI Antibody Core Code: A0784
"Lot" Number: 2 Position: 100 (1185-1284)
% Identical Homology of Target to Mouse Protein: 43% (NP_061274.2| synaptotagmin 11)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
EPTSGKIYTATGEVDQATGEPLYAATQVDPESGEVYTKLA
RVDPKTGKIVIVRILLISKTDERGRPEEIDPSTCEIDPVS
GRVLKFFNKTVYVYNMIDPV

Sequence Name: coracle
Full name: coracle
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 1698
NCBI information page:

Full length sequence:
MPAEIKPSAPAEPETPTKSKPKSSSSSHGKPALARVTLLDGSLLDVSIDR
KAIGRDVINSICAGLNLIEKDYFGLTYETPTDPRTWLDLEKPVSKFFRTD
TWPLTFAVKFYPPEPSQLKEDITRYHLCLQVRNDILEGRLPCTFVTHALL
GSYLVQSEMGDYDAEEMPTRAYLKDFKIAPNQTAELEDKVMDLHKTHKGQ
SPAEAELHYLENAKKLAMYGVDLHPAKDSEGVDIMLGVCASGLLVYRDKL
RINRFAWPKILKISYKRHHFYIKIRPGEFEQYESTIGFKLANHRAAKKLW
KSCVEHHTFFRLMTPEPVSKSKMFPVFGSTYRYKGRTQAESTNTPVDRTP
PKFNRTLSGARLTSRSMDALALAEKEKVARKSSTLDHRGDRNADGDAHSR
SPIKNKKEKDADKEAKLREKKQKEKEEKERKEREKRELEEKKKAEKAAKA
ALAAGAAAGAAVNGNDELNDSNKSDKSSGRRGVGIFSSGRKSKSGSPSKD
GKDKSGKDKDKEVGRLGLVVTSGLGDNQQDQNLDEAARNAAKNRGSTTPG
VTRQYEYAVDNDGNTSPTRKSYTPGGFRYDQDPNSRKSGADGQEQLSPTS
QQKKIGLAFNYAPGNENALKETAEKLKAGQLSPRTQDKLNRGQLSPKSRA
KLLQDPLLSPTTRAKLQGSAVDAAAVPLSDSQKRSYSPTKGPQGYSSGAP
GSYKPISDPTADFLESQRYNKEPGYVGPSKADVAAGLAGAAGSKKPGSPT
KTGKGAPGAAAAAAAGAAGAAAAAAKPKKRRVKIMVITSKFDPSTKRIDA
ENGSIEHSTGILDPATGLIDTKYGVIDPKKGTLEALNTKTGKKEVFQGDV
DGKTGNLHLVSGVADPKTGRLDDTLGQIVCITPQDNPVVELTVITSRIDP
ATGKIDTVNGDVERSLGVLNLDTGLLDTKYGEINTRTGELKAIDPKSGKI
VVSKNVKVDPGTGQITILGIVDPKTNKIDPNQGRLIEVGQQIDPIVEVTS
LAGKFDSKRNIIDPKTAQVETSGGQFDPKAGKIDTKYGQIDLVKHTITFN
DPKSGKTVTRDIKIEPTTGQIVLKNQVNPKNNKPDKDYARIISLRIVQQR
VDPATKAPITEVSASKDKDIVVDPKSNQIWVPTGATDPATKEQQYISSSV
DPKTGYVITIYGYLDPKTNEIKKQTKLDPNTIKIEPTSGKIYTATGEVDQ
ATGEPLYAATQVDPESGEVYTKLARVDPKTGKIVIVRILLISKTDERGRP
EEIDPSTCEIDPVSGRVLKFFNKTVYVYNMIDPVTGEIVQVDPNDPRFAG
ARTTVTHTMTLTGEIDPVTGRIKSEYGDIDPNTGDIDPATAVTDPVTGKL
ILNYAQIDPSHFGKQAQVQTTTETVPITRQQFFDGVKHISKGALRRDSEG
SSDDDMTAQYGADQVNEILIGSPAGQAGGKLGKPVSTPTVVKTTTKQVLT
KNIDGVTHNVEEEVRNLGTGEVTYSTQEHKADATPTDLSGAYVTATAVTT
RTATTHEDLGKNAKTEQLEEKTVATTRTHDPNKQQQRVVTQEVKTTATVT
SGDQYQRRDSVSSTSSGDSGTPIDGPYDGASVVRTDNQKSPLFTTSATTG
PHVESTRVVLGEDTPGFSGHGEIISTQTVSSKTRTVETITYKTERDGIVE
TRVEQKITIQSDGDPIDHDKALAEAIQEATAMNPDMTVEKIEIQQQTQ