fruit fly ( Drosophila melanogaster )
dlp

Western result: +

GST GST-dlp (fragment)

33.16 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: dlp
CBI Antibody Core Code: A0900
"Lot" Number: 1 Position: 56(91-146)
% Identical Homology of Target to Mouse Protein: 37% (NP_057905.1| glypican 1)
Molecular Weight of fusion protein: 33.16 KDa

Target sequence used to make this antibody:
GIDAIEIPQKPSNERVLRYCESPSVGTCCTYNMETRMAMQ
SRQQLEGHTKDQISRM

Sequence Name: dlp
Full name: dally-like
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 765
NCBI information page:

Full length sequence:
MLHQQQQQQHLHCRRKATATTTARLVIFSSPLLLLLLTTHLPPTLQADNG
PAPQVAALAAPNPAGGVAGSSIIDQFSPNCSAVTHIFQARGIDAIEIPQK
PSNERVLRYCESPSVGTCCTYNMETRMAMQSRQQLEGHTKDQISRMSGIL
GSKATKFKDIFTALLKESRTQFNSMFIRTYGVIYERNSYVFSDLFKELET
YFANGRVDLLEVMDKFFNTLYQKMFTVLNTQYTFDENYMRCVSEHMKELK
PFGDVPDKLSVQIKRSFVATRTYGQALTTASEVAKKVLNVRLNADCTGAL
TKMQHCGACKGYTEKPCTNYCVNVIKGCLHYQHEFDSEWENFAMAMDKVA
ERLLGSFNIVMVVEPLNIKISEAIMNFQDSGQDITNRVFQGCGRPKLKKM
KRSISPKLQGVQILNARSPVEADTLDIDETLDEAIVLRERRAAEPGSQET
SAQQSQEQGVGKSGNGGGGGGGNNRRQQQRRKQQQQRRKQQNNRDDNDDD
DNESGGGREPILDRIVRDIRQRVKDYKKFWSNLPHSVCSNEDIASSSDVD
GMCWNGHTIDRYMHSITTEHGSNPEFTGNPASTKQTAQMASQLSHLKNAI
VHLRNAYNGQDVEWSEQEELPYAGSGAGSGSGSEDDEDDDEGSGLGPFEP
SHKPDVERPSVDADNDDDEDAGGRGHMPTHTSRPTSGVDDKNPLIHTTHF
DQDHNDLDEDHRQLDEDEDTDAGHDGANDNRSSDAPEKMTLRRALVVYLL
PLYMAWFGGVCADLL