fruit fly ( Drosophila melanogaster )
fz

Western result: + ???

GST GST-fz (fragment)

38 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: fz
CBI Antibody Core Code: A0901
"Lot" Number: 1 Position: 100(135-234)
% Identical Homology of Target to Mouse Protein: 34% (NP_032083.2| frizzled 7)
Molecular Weight of fusion protein: 38 KDa

Target sequence used to make this antibody:
EKLMKTYNFNWPENLECSKFPVHGGEDLCVAENTTSSAST
AATPTRSVAKVTTRKHQTGVESPHRNIGFVCPVQLKTPLG
MGYELKVGGKDLHDCGAPCH

Sequence Name: fz
Full name: frizzled
UniGene Number: N.A.
Accession Number: N.A.
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 581
NCBI information page:

Full length sequence:
MWRQILFILPTLIQGVQRYDQSPLDASPYYRSGGGLMASSGTELDGLPHH
NRCEPITISICKNIPYNMTIMPNLIGHTKQEEAGLEVHQFAPLVKIGCSD
DLQLFLCSLYVPVCTILERPIPPCRSLCESARVCEKLMKTYNFNWPENLE
CSKFPVHGGEDLCVAENTTSSASTAATPTRSVAKVTTRKHQTGVESPHRN
IGFVCPVQLKTPLGMGYELKVGGKDLHDCGAPCHAMFFPERERTVLRYWV
GSWAAVCVASCLFTVLTFLIDSSRFRYPERAIVFLAVCYLVVGCAYVAGL
GAGDSVSCREPFPPPVKLGRLQMMSTITQGHRQTTSCTVLFMALYFCCMA
AFAWWSCLAFAWFLAAGLKWGHEAIENKSHLFHLVAWAVPALQTISVLAL
AKVEGDILSGVCFVGQLDTHSLGAFLILPLCIYLSIGALFLLAGFISLFR
IRTVMKTDGKRTDKLERLMLRIGFFSGLFILPAVGLLGCLFYEYYNFDEW
MIQWHRDICKPFSIPCPAARAPGSPEARPIFQIFMVKYLCSMLVGVTSSV
WLYSSKTMVSWRNFVERLQGKEPRTRAQAYV