fruit fly ( Drosophila melanogaster )
wun2

Western result: +

GST GST-wun2 (fragment)

23.9 kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: wun2
CBI Antibody Core Code: A0955
"Lot" Number: 1 Position: 90 (1-90)
% Identical Homology of Target to Mouse Protein: 0%
Molecular Weight of fusion protein: 23.9 KDa

Target sequence used to make this antibody:
MSTLRPVSVCDTTPLQRFESQSSSGEEPSSPTASSIIAAA
SGPNHNNNNNVKLDLQLPTFVDSTRSQGSLYAVASNKKPP
LRGPRQIFGR

Sequence Name: wun2
Full name: wunen2
UniGene Number: N.A.
Accession Number: NP_524991
Source organism: fruit fly ( Drosophila melanogaster )
Full length size (aa): 350
NCBI information page:

Full length sequence:
MSTLRPVSVCDTTPLQRFESQSSSGEEPSSPTASSIIAAASGPNHNNNNN
VKLDLQLPTFVDSTRSQGSLYAVASNKKPPLRGPRQIFGRILTDLCLLSC
VGLPMLGFSLWGEAVKRGFFCDDSSLRHPYRDSTMPSWILYLMCGALPLT
VMLVVEFFRGQDKRLHSPFPKSTMCSGYHLCHLELPTWLVECYHRMGIFI
FGLGVEQLSTNIAKYSIGRLRPHFYTLCQPVMKDGTTCSDPINAARYIEE
FTCAAVDITSKQLKDMRLSFPSGHASFACYSMLYLVIYLHRRMQWKQLRM
LCHLLQFLLLMFAWYTALTRVSDYKHHWSDVLAGSGIGLTYAVVVTSTMW