chicken ( Gallus gallus )
Topo II-alpha

Antibody Name/Abbreviation: Topo II-alpha
CBI Antibody Core Code: A0587
"Lot" Number: 3 Position: 100(641-740)
% Identical Homology of Target to Mouse Protein: 87% (NP_035753.1| topoisomerase (DNA) II alpha; DNA Topoisomerase II alpha)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
YSGPEDDAAITLAFSKKKVEERKEWLTNFMEDRRQRNVHG
LPEDYLYGKDTNYLTYNDFINKELVLFSNSDNERSIPSLV
DGLKPGQRKVLFTCFKRNDK

Other western:
Code: A0587
Lot: 2

Western result: -

GST GST-Topo II-alpha (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Sequence Name: Topo II-alpha
Full name: DNA topoisomerase II, alpha isozyme.
UniGene Number: N.A.
Accession Number: O42130
Source organism: chicken ( Gallus gallus )
Full length size (aa): 1553
NCBI information page: click here

Full length sequence:
MELLDSPAPLRPLHDNPRLPKADGAQKRLSVERIYQKKTQLEHILLRPDT
YIGSVETVTQQMWVFDEDVGLNCRDVTFVPGLYKIFDEILVNAADNKQRD
KSMSCIKVTIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSS
NYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACREYKKLFKQTWTDNMG
KAGEMTLKHFDGEDYTCVTFQPDLSKFKMTILDKDIVALMSRRAYDIAGS
TKDVKVFLNGKRLPVKGFRSYVDLYLKDKVDETGNALKVIHEEVNSRWEV
CLTLSEKGFQQVSFVNSIATTKGGRHVDYVADQIVTKLIDVVKKKNKNGV
GVKPFQVKNHMWIFVNSLIENPTFDSQTKENMTLQAKSFGSTCKLSEKFI
KGAVGCGIVESILNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGS
KNSIDCTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKMLNVREASHK
QIMENAEINNIIKIVGLQYKKNYEDRESLKSLRYGKIMIMTDQDQDGSHI
KGLLINFIHHNWPSLLRHNFLEEFITPIIKVSKNKEEIPFYSIPEFEEWK
SSTQNYNSWKIKYYKGLGTSTSKEAKEYFADMARHRIGFKYSGPEDDAAI
TLAFSKKKVEERKEWLTNFMEDRRQRNVHGLPEDYLYGKDTNYLTYNDFI
NKELVLFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKGAQLAG
SVAEMSSYHHGEASLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKD
SASPRYIFTMLSPLARLLFPPVDDNVLRFLYDDNQRVEPEWYMPIIPMVL
INGAEGIGTGWSCKIPNFDIRETVNNIRCLLDGKEPLPMLPSYKNFKGTI
DELGPNQYVISGEVSILDSTTIEITELPVRTWTQTYKEQVLEPMLNGTEK
TPPLITDYKEYHTDTTVKFVVKMSEEKLAEAEAVGLHKVFKLQTNLTCNS
MVLFDHVGFLKKYESPQDILKEFFELRLRYYGLRKEWLIGMLGAESAKLN
NQARFILEKIDGKIVIENKPKKELIQVLIQRGYESDPVKAWKELQNKEEE
EGDESGEESAAATGPDFNYLLNMPLWYLTKEKKDELCKQRDNKDKELEDL
KHKSPSDLWKEDLAAFVEELDAVEAKQMQDEMAGITGKPLKVKGGKQGGK
QKVTKAQLAEVMPSPHGIRVVPRVTAEMKAEAEKRIKKKIKSEKNESDEK
QEGNSSGDKEPSSLKQRLAQKRKAEQGTKRQTTLPFKPIKKMKRNPWSDS
ESDSESDDFEVPSKRERVVRQAAAKIKPMVNSDSDADLTSSDEDSEYQEN
SEGNTDSDTTSKKKPPKAKAVPKEKKGKAPKEKPLPDAVPVRVQNVAAES
ASQDPAAPPVSVPRAQAVPKKPAAAKKGSTAKDNQPSIMDILTKKKAAPK
APRRAQREESPPSEATAAVAKKPGPPRGRKATKRLTSSSDSDSDFGSRPS
KSVAAKKSKRDDDDSYSIDLTADSPAAAAPRTRPGRLKKPVQYLESSDED
DMF