chicken ( Gallus gallus )
Topo II-beta

Western result: +

GST GST-Topo II-beta (fragment)

N.A. kDa (recombinant)

The western blot contains a test lane of ~20ug of a total protein extract from E coli with ~50ng to 500ng of the antigen (GST-antigen fusion protein). The negative control lane is the same except it contains ~50ng to 100 ng of a GST fusion protein of an irrelevant antigen. The blot was probed with a final antisera dilution of 1:1,000. The secondary antibody (Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated (Pierce)) is used at 1:5,000 dilution. Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.

Antibody Name/Abbreviation: Topo II-beta
CBI Antibody Core Code: A0588
"Lot" Number: 1 Position: 100(115-214)
% Identical Homology of Target to Mouse Protein: 99% (NP_033435.2| topoisomerase (DNA) II beta)
Molecular Weight of fusion protein: N.A. KDa

Target sequence used to make this antibody:
LVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVV
EHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAK
LCNIFSTKFTVETACKEYKH

Sequence Name: Topo II-beta
Full name: DNA topoisomerase II, beta isozyme
UniGene Number: N.A.
Accession Number: O42131
Source organism: chicken ( Gallus gallus )
Full length size (aa): 1627
NCBI information page: click here

Full length sequence:
MAKSGGGGGGGGGGGGGGGGSGGLTCVTLFDNQINASKKEESESVNKNDT
SKKMSVERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVGMNCRE
VTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKG
IPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFS
TKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLS
KFKMENLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLY
VKDKLDETGVALKVIHEVVNERWDVCLTLSEKGFQQISFVNSIATTKGGR
HVDYVVDQVVGKLIEVVKKKNKAGVSVKPFQVKNHIWVFVNCLIENPSFD
SQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLN
KKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLG
VIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYED
PESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFI
TPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEA
KEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQ
RRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKP
GQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQN
FVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDN
LLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVN
NVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEIT
ELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTE
EKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFD
LRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLI
QMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNY
ILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEE
LEKVEAQEREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVPQ
ITSAMKADASRKLLKKKKGDADSVAIKMEFDEEFGGVQAEGGGDDTVNTA
ASGTKTPKLKREKKEPGTRVRRAPSSTKSSAKKVKKRNPWSDDESKSESD
LEESEPVIIPRDSLLRRAAADRAKYTFDFSKEEDDAHDDDDANNNNDLDE
LKVKASPVINDREDEFVPSDSVEKDEYDFSPVKSKPSPEKMSQEKKNQDF
GNIFSFPSYSQKTDDDTTKLDSDEEDSTPVFSSPFAPKQTEKMLSKTVAA
KKAKVDVPPKPKRAPKAKKMETVNSDSDSEFGIPKKTAAPKGKGRGAKKR
KTSGSENEGEYNPGKKAPKSTPCKKSKKAAFDQDSDVEIFQSGFASETAP
KPRTGRARKEVKYFAESDEDDDFDMFN